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Journal of Food Technology

ISSN: Online 1993-6036
ISSN: Print 1684-8462
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Production of Alpha Amylase by a Thermophilic Strain of Bacillus Licheniformis

Hamad Ashraf , Kokub Rana , Hifzah Zainab and Ikram-ul-Haq
Page: 64-67 | Received 21 Sep 2022, Published online: 21 Sep 2022

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Abstract

The production of alpha amylase was carried out by thermophilic strains of Bacillus licheniformis. The isolated cultures were screened by solid-state fermentation using wheat bran as substrate, moistened with phosphate buffer (pH 7.5). The culture GHB8 that gave the maximum production of alpha amylase (1277 U/g/min) was used for further studies. The maximum production of alpha amylase (1630 U/g/min) was reached when wheat bran was partially replaced with cottonseed meal in the ratio of 3:1 along with 1 per cent starch. The incubation temperature 40oC was found to be optimum for the production of alpha amylase. The enzyme was most active between 60-70?C.


How to cite this article:

Hamad Ashraf , Kokub Rana , Hifzah Zainab and Ikram-ul-Haq . Production of Alpha Amylase by a Thermophilic Strain of Bacillus Licheniformis.
DOI: https://doi.org/10.36478/jftech.2005.64.67
URL: https://www.makhillpublications.co/view-article/1684-8462/jftech.2005.64.67