Hamad Ashraf  , Kokub Rana  , Hifzah Zainab  , Ikram-ul-Haq  , 
    Production of Alpha Amylase by a Thermophilic Strain of Bacillus Licheniformis,
    Journal of Food Technology,
    Volume 3,Issue 1,
    2005,
    Pages 64-67,
    ISSN 1684-8462,
    jftech.2005.64.67,
    (https://makhillpublications.co/view-article.php?doi=jftech.2005.64.67)
    Abstract: The production of alpha amylase was carried out by thermophilic strains of Bacillus licheniformis. The isolated cultures were screened by solid-state fermentation using wheat bran as substrate, moistened with phosphate buffer (pH 7.5). The culture GHB8 that gave the maximum production of alpha amylase (1277 U/g/min) was used for further studies. The maximum production of alpha amylase (1630 U/g/min) was reached when wheat bran was partially replaced with cottonseed meal in the ratio of 3:1 along with 1 per cent starch. The incubation temperature 40oC was found to be optimum for the production of alpha amylase. The enzyme was most active between 60-70?C.
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