@article{MAKHILLJFT2005317908, title = {Production of Alpha Amylase by a Thermophilic Strain of Bacillus Licheniformis}, journal = {Journal of Food Technology}, volume = {3}, number = {1}, pages = {64-67}, year = {2005}, issn = {1684-8462}, doi = {jftech.2005.64.67}, url = {https://makhillpublications.co/view-article.php?issn=1684-8462&doi=jftech.2005.64.67}, author = {Hamad Ashraf,Kokub Rana,Hifzah Zainab and}, keywords = {}, abstract = {The production of alpha amylase was carried out by thermophilic strains of Bacillus licheniformis. The isolated cultures were screened by solid-state fermentation using wheat bran as substrate, moistened with phosphate buffer (pH 7.5). The culture GHB8 that gave the maximum production of alpha amylase (1277 U/g/min) was used for further studies. The maximum production of alpha amylase (1630 U/g/min) was reached when wheat bran was partially replaced with cottonseed meal in the ratio of 3:1 along with 1 per cent starch. The incubation temperature 40oC was found to be optimum for the production of alpha amylase. The enzyme was most active between 60-70?C.} }