TY - JOUR T1 - Production of Alpha Amylase by a Thermophilic Strain of Bacillus Licheniformis AU - , Hamad Ashraf AU - , Kokub Rana AU - , Hifzah Zainab AU - , Ikram-ul-Haq JO - Journal of Food Technology VL - 3 IS - 1 SP - 64 EP - 67 PY - 2005 DA - 2001/08/19 SN - 1684-8462 DO - jftech.2005.64.67 UR - https://makhillpublications.co/view-article.php?doi=jftech.2005.64.67 KW - AB - The production of alpha amylase was carried out by thermophilic strains of Bacillus licheniformis. The isolated cultures were screened by solid-state fermentation using wheat bran as substrate, moistened with phosphate buffer (pH 7.5). The culture GHB8 that gave the maximum production of alpha amylase (1277 U/g/min) was used for further studies. The maximum production of alpha amylase (1630 U/g/min) was reached when wheat bran was partially replaced with cottonseed meal in the ratio of 3:1 along with 1 per cent starch. The incubation temperature 40oC was found to be optimum for the production of alpha amylase. The enzyme was most active between 60-70?C. ER -