Research Journal of Medical Sciences
ISSN: Online 1993-6095ISSN: Print 1815-9346
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Development of a Domestic Real-Time Polymerase Chain Reaction (PCR) Test System for the Identification of Strains of Yersinia enterocolitica and Yersinia pseudotuberculosis
Gulzhagan Chuzhebaeva, Beishova Indira, VadimUlyanov , Kokanov Sabi, Zhumagul Kirkimbaeva, Svetlana Yermagambetova and Batyrzan Mustafin
Page: 199-204 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
Pseudotuberculosis and intestinal yersiniosis are among the relatively new infectious diseases that have been particularly intensively studied in the past 25-30 years. Intestinal yersiniosis refers to food zoonoses, i.e., to diseases, the source of infection in which are sick wild, agricultural, domestic animals and the transmission factors-food: milk, meat, vegetables and fruits. The wide distribution of iersiniosis, the variety of the clinic and the laboriousness involved in making the diagnosis made the problem of intestinal iersiniasis on a global scale urgent. The Polymerase Chain Reaction (PCR) method is more sensitive and specific in comparison with microbiological methods and its modification in the Real-time format using a hybridization probe with fluorescent The label allows you to quickly analyze the results without conducting electrophoresis.
How to cite this article:
Gulzhagan Chuzhebaeva, Beishova Indira, VadimUlyanov , Kokanov Sabi, Zhumagul Kirkimbaeva, Svetlana Yermagambetova and Batyrzan Mustafin. Development of a Domestic Real-Time Polymerase Chain Reaction (PCR) Test System for the
Identification of Strains of Yersinia enterocolitica and Yersinia pseudotuberculosis.
DOI: https://doi.org/10.36478/rjmsci.2020.199.204
URL: https://www.makhillpublications.co/view-article/1815-9346/rjmsci.2020.199.204