TY - JOUR
T1 - Development of a Domestic Real-Time Polymerase Chain Reaction (PCR) Test System for the
Identification of Strains of Yersinia enterocolitica and Yersinia pseudotuberculosis
AU - Indira, Beishova AU - Chuzhebaeva, Gulzhagan AU - , VadimUlyanov AU - Sabi, Kokanov AU - Kirkimbaeva, Zhumagul AU - Yermagambetova, Svetlana AU - Mustafin, Batyrzan
JO - Research Journal of Medical Sciences
VL - 14
IS - 6
SP - 199
EP - 204
PY - 2020
DA - 2001/08/19
SN - 1815-9346
DO - rjmsci.2020.199.204
UR - https://makhillpublications.co/view-article.php?doi=rjmsci.2020.199.204
KW - Yersinia enterocolitica
KW -Yersinia pseudotuberculosis
KW -real time PCR
KW -primers and probe
AB - Pseudotuberculosis and intestinal yersiniosis
are among the relatively new infectious diseases that have
been particularly intensively studied in the past 25-30
years. Intestinal yersiniosis refers to food zoonoses, i.e.,
to diseases, the source of infection in which are sick wild,
agricultural, domestic animals and the transmission
factors-food: milk, meat, vegetables and fruits. The wide
distribution of iersiniosis, the variety of the clinic and the
laboriousness involved in making the diagnosis made the
problem of intestinal iersiniasis on a global scale urgent.
The Polymerase Chain Reaction (PCR) method is more
sensitive and specific in comparison with microbiological
methods and its modification in the Real-time format
using a hybridization probe with fluorescent The label
allows you to quickly analyze the results without
conducting electrophoresis.
ER -