TY - JOUR T1 - Development of a Domestic Real-Time Polymerase Chain Reaction (PCR) Test System for the Identification of Strains of Yersinia enterocolitica and Yersinia pseudotuberculosis AU - Indira, Beishova AU - Chuzhebaeva, Gulzhagan AU - , VadimUlyanov AU - Sabi, Kokanov AU - Kirkimbaeva, Zhumagul AU - Yermagambetova, Svetlana AU - Mustafin, Batyrzan JO - Research Journal of Medical Sciences VL - 14 IS - 6 SP - 199 EP - 204 PY - 2020 DA - 2001/08/19 SN - 1815-9346 DO - rjmsci.2020.199.204 UR - https://makhillpublications.co/view-article.php?doi=rjmsci.2020.199.204 KW - Yersinia enterocolitica KW -Yersinia pseudotuberculosis KW -real time PCR KW -primers and probe AB - Pseudotuberculosis and intestinal yersiniosis are among the relatively new infectious diseases that have been particularly intensively studied in the past 25-30 years. Intestinal yersiniosis refers to food zoonoses, i.e., to diseases, the source of infection in which are sick wild, agricultural, domestic animals and the transmission factors-food: milk, meat, vegetables and fruits. The wide distribution of iersiniosis, the variety of the clinic and the laboriousness involved in making the diagnosis made the problem of intestinal iersiniasis on a global scale urgent. The Polymerase Chain Reaction (PCR) method is more sensitive and specific in comparison with microbiological methods and its modification in the Real-time format using a hybridization probe with fluorescent The label allows you to quickly analyze the results without conducting electrophoresis. ER -