@article{MAKHILLRJMS202014612268, title = {Development of a Domestic Real-Time Polymerase Chain Reaction (PCR) Test System for the Identification of Strains of Yersinia enterocolitica and Yersinia pseudotuberculosis}, journal = {Research Journal of Medical Sciences}, volume = {14}, number = {6}, pages = {199-204}, year = {2020}, issn = {1815-9346}, doi = {rjmsci.2020.199.204}, url = {https://makhillpublications.co/view-article.php?issn=1815-9346&doi=rjmsci.2020.199.204}, author = {Beishova,Gulzhagan,VadimUlyanov,Kokanov,Zhumagul,Svetlana and}, keywords = {Yersinia enterocolitica,Yersinia pseudotuberculosis,real time PCR,primers and probe}, abstract = {Pseudotuberculosis and intestinal yersiniosis are among the relatively new infectious diseases that have been particularly intensively studied in the past 25-30 years. Intestinal yersiniosis refers to food zoonoses, i.e., to diseases, the source of infection in which are sick wild, agricultural, domestic animals and the transmission factors-food: milk, meat, vegetables and fruits. The wide distribution of iersiniosis, the variety of the clinic and the laboriousness involved in making the diagnosis made the problem of intestinal iersiniasis on a global scale urgent. The Polymerase Chain Reaction (PCR) method is more sensitive and specific in comparison with microbiological methods and its modification in the Real-time format using a hybridization probe with fluorescent The label allows you to quickly analyze the results without conducting electrophoresis.} }