Beishova  Indira, Gulzhagan  Chuzhebaeva, VadimUlyanov  , Kokanov  Sabi, Zhumagul  Kirkimbaeva, Svetlana  Yermagambetova, Batyrzan  Mustafin, 
    Development of a Domestic Real-Time Polymerase Chain Reaction (PCR) Test System for the
Identification of Strains of <i>Yersinia enterocolitica</i> and <i>Yersinia pseudotuberculosis</i>,
    Research Journal of Medical Sciences,
    Volume 14,Issue 6,
    2020,
    Pages 199-204,
    ISSN 1815-9346,
    rjmsci.2020.199.204,
    (https://makhillpublications.co/view-article.php?doi=rjmsci.2020.199.204)
    Abstract: Pseudotuberculosis and intestinal yersiniosis
are among the relatively new infectious diseases that have
been particularly intensively studied in the past 25-30
years. Intestinal yersiniosis refers to food zoonoses, i.e.,
to diseases, the source of infection in which are sick wild,
agricultural, domestic animals and the transmission
factors-food: milk, meat, vegetables and fruits. The wide
distribution of iersiniosis, the variety of the clinic and the
laboriousness involved in making the diagnosis made the
problem of intestinal iersiniasis on a global scale urgent.
The Polymerase Chain Reaction (PCR) method is more
sensitive and specific in comparison with microbiological
methods and its modification in the Real-time format
using a hybridization probe with fluorescent The label
allows you to quickly analyze the results without
conducting electrophoresis.
    Keywords: Yersinia enterocolitica;Yersinia pseudotuberculosis;real time PCR;primers and probe