Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
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Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Visual Detection of Porcine Epidemic Diarrhea Virus
Xu Tong, Chang Bao, Ge Jian, Wang Jin-Liang, Yang Cui-Jun, Wu Shu-Qin and Sun Quan-Wen
Page: 1897-1900 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
To develop a new, rapid, practical and sensitive method for detection of Porcine Epidemic Diarrhea Virus (PEDV), a Reverse Transcription Loop Mediated isothermal Amplification (RT-LAMP) assay was established based on six primers in the conserved gene. The method had specificity but no cross-reaction with other viruses and was 100 fold higher than RT-PCR. The result suggested that the newly developed RT-LAMP assay is a simple and specific method for rapid detection of PEDV in field conditions and it has a high practical value in PEDV integrated control and early diagnosis.
How to cite this article:
Xu Tong, Chang Bao, Ge Jian, Wang Jin-Liang, Yang Cui-Jun, Wu Shu-Qin and Sun Quan-Wen. Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Visual Detection of Porcine Epidemic Diarrhea Virus.
DOI: https://doi.org/10.36478/javaa.2012.1897.1900
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2012.1897.1900