TY  - JOUR
T1  - Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Visual Detection of Porcine Epidemic Diarrhea Virus
AU - Tong, Xu AU - Bao, Chang AU - Jian, Ge AU - Jin-Liang, Wang AU - Cui-Jun, Yang AU - Shu-Qin, Wu AU - Quan-Wen, Sun 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 11
SP  - 1897
EP  - 1900
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1897.1900
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1897.1900
KW  - China
KW  -virus
KW  -diagnosis
KW  -detection methods
KW  -LAMP
KW  -PEDV
AB  - To develop a new, rapid, practical and sensitive method for detection of Porcine Epidemic Diarrhea Virus (PEDV), a Reverse Transcription Loop Mediated isothermal Amplification (RT-LAMP) assay was established based on six primers in the conserved gene. The method had specificity but no cross-reaction with other viruses and was 100 fold higher than RT-PCR. The result suggested that the newly developed RT-LAMP assay is a simple and specific method for rapid detection of PEDV in field conditions and it has a high practical value in PEDV integrated control and early diagnosis.
ER  -