@article{MAKHILLJAVA201211113483,
    title = {Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Visual Detection of Porcine Epidemic Diarrhea Virus},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {11},
    pages = {1897-1900},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.1897.1900},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.1897.1900},
    author = {Xu,Chang,Ge,Wang,Yang,Wu and},
    keywords = {China,virus,diagnosis,detection methods,LAMP,PEDV},
    abstract = {To develop a new, rapid, practical and sensitive method for detection of Porcine Epidemic Diarrhea Virus (PEDV), a Reverse Transcription Loop Mediated isothermal Amplification (RT-LAMP) assay was established based on six primers in the conserved gene. The method had specificity but no cross-reaction with other viruses and was 100 fold higher than RT-PCR. The result suggested that the newly developed RT-LAMP assay is a simple and specific method for rapid detection of PEDV in field conditions and it has a high practical value in PEDV integrated control and early diagnosis.}
    }