Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
Abstract
To explore effects of intensity and time of cold stress on expression of Hsp70 gene in blood Lymphocyte of piglets, Western-blotting and real-time PCR were performed to detect expression level of HSP70 gene, respectively. The results showed as followed: at 10 to 4°C, HSP70 expression increased significantly for 0.5 h and subsequently decreased slightly before increasing markedly again at both 6 and 24 h; HSP70 mRNA expression level increased sharply after piglets were exposed for 0.5 h. The condition maintained for 6 h then declined at 12 h it reached a level apparently higher than the level prior to cold exposure at 24 h the transcription level recovered to normal. At 3 to 3°C the expression level increased at 6 h but then returned to pre-exposure levels; mRNA increased significantly at 12 h. At 4-10°C, no marked difference in expression level was detected. In conclusion, HSP70 mRNA expression showed the faster regular pattern therefore, it can be used as an objective parameter to evaluate animal cold stress conditions.
How to cite this article:
Huan Min Yang, Shi Ze Li, Jian Fa Wang, Jing Ru Guo, Yong Kui Wu, Hong Ji and Ju Xiong Liu. Effects of Cold Stress on Expression of Hsp70 Gene in Blood Lymphocyte of Piglets.
DOI: https://doi.org/10.36478/javaa.2012.3914.3920
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2012.3914.3920