Huan Min  Yang, Shi Ze  Li, Jian Fa  Wang, Jing Ru  Guo, Yong Kui  Wu, Hong  Ji, Ju Xiong  Liu, 
    Effects of Cold Stress on Expression of <I>Hsp70</I> Gene in Blood Lymphocyte of Piglets,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 21,
    2012,
    Pages 3914-3920,
    ISSN 1680-5593,
    javaa.2012.3914.3920,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.3914.3920)
    Abstract: To explore effects of intensity and time of cold stress on expression of <I>Hsp70</I> gene in blood Lymphocyte of piglets, Western-blotting and real-time PCR were performed to detect expression level of <I>HSP70</I> gene, respectively. The results showed as followed: at &#61473;10 to &#61473;4&deg;C, HSP70 expression increased significantly for 0.5 h and subsequently decreased slightly before increasing markedly again at both 6 and 24 h; HSP70 mRNA expression level increased sharply after piglets were exposed for 0.5 h. The condition maintained for 6 h then declined at 12 h it reached a level apparently higher than the level prior to cold exposure at 24 h the transcription level recovered to normal. At &#61473;3 to 3&deg;C the expression level increased at 6 h but then returned to pre-exposure levels; mRNA increased significantly at 12 h. At 4-10&deg;C, no marked difference in expression level was detected. In conclusion, HSP70 mRNA expression showed the faster regular pattern therefore, it can be used as an objective parameter to evaluate animal cold stress conditions.
    Keywords: transcription;HSP70 mRNA;HSP70;cold stress;Piglet;animal