TY  - JOUR
T1  - Effects of Cold Stress on Expression of <I>Hsp70</I> Gene in Blood Lymphocyte of Piglets
AU - Yang, Huan Min AU - Li, Shi Ze AU - Wang, Jian Fa AU - Guo, Jing Ru AU - Wu, Yong Kui AU - Ji, Hong AU - Liu, Ju Xiong 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 21
SP  - 3914
EP  - 3920
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3914.3920
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3914.3920
KW  - transcription
KW  -HSP70 mRNA
KW  -HSP70
KW  -cold stress
KW  -Piglet
KW  -animal
AB  - To explore effects of intensity and time of cold stress on expression of <I>Hsp70</I> gene in blood Lymphocyte of piglets, Western-blotting and real-time PCR were performed to detect expression level of <I>HSP70</I> gene, respectively. The results showed as followed: at &#61473;10 to &#61473;4&deg;C, HSP70 expression increased significantly for 0.5 h and subsequently decreased slightly before increasing markedly again at both 6 and 24 h; HSP70 mRNA expression level increased sharply after piglets were exposed for 0.5 h. The condition maintained for 6 h then declined at 12 h it reached a level apparently higher than the level prior to cold exposure at 24 h the transcription level recovered to normal. At &#61473;3 to 3&deg;C the expression level increased at 6 h but then returned to pre-exposure levels; mRNA increased significantly at 12 h. At 4-10&deg;C, no marked difference in expression level was detected. In conclusion, HSP70 mRNA expression showed the faster regular pattern therefore, it can be used as an objective parameter to evaluate animal cold stress conditions.
ER  -