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Journal of Animal and Veterinary Advances

ISSN: Online 1993-601X
ISSN: Print 1680-5593
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Molecular Analysis of Infectious Bronchitis Viruses Isolated in Iran from 1998-2008

N. Ghahremani, M.H. Bozorgmehri Fard, H. Shoushtari, R. Momayez, N. Sheikhi, A. Khoshzahmat and F. Eshratabadi
Page: 2961-2967 | Received 21 Sep 2022, Published online: 21 Sep 2022

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https://doi.org/10.36478/javaa.2011.2961.2967

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Abstract

Ten isolates of Infectious Bronchitis Virus (IBV) were detected from the trachea and lung of IB suspected chickens from 1998-2008 in Iran by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). For further analysis, the entire S1 gene of the IBV isolates amplified by other RT-PCR. Phylogenetic analysis based on both S1 gene nucleotide and amino acid deduced sequences showed that isolates of Iran were classified into two genotypes Massachusetts (3 isolates) and 4/91 (7 isolates). Three isolates of Massachusetts serotype had very strong genetic relatedness to Massachusetts-type vaccines (particularly H120) indicating the probability of the detection and isolation of vaccine strains. The present study demonstrates that 4/91 type has been circulating in the country since 1998. Comparison of 4/91 isolates of Iran and almost all foreign isolates of 4/91 showed that Iranian 4/91 isolates (except for IR/491/08) had maximum average nucleotide and amino acid identities with French isolate FR-94047-94, supporting this probability that 4/91 isolates of Iran originated from France. The isolate IR/491/08 recovered from flock vaccinated with 4/91 vaccine strain and had high sequence similarity of the S1 gene with 4/91 vaccine strain. Therefore, it is likely that this isolate was re-isolation vaccine strain. The results also revealed that the application of Massachusetts vaccines may not contain the replication of the 4/91 type.

How to cite this article:

N. Ghahremani, M.H. Bozorgmehri Fard, H. Shoushtari, R. Momayez, N. Sheikhi, A. Khoshzahmat and F. Eshratabadi. Molecular Analysis of Infectious Bronchitis Viruses Isolated in Iran from 1998-2008.
DOI: https://doi.org/10.36478/javaa.2011.2961.2967
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2011.2961.2967