@article{MAKHILLJAVA201110223041,
    title = {Molecular Analysis of Infectious Bronchitis Viruses Isolated in Iran from 1998-2008},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {10},
    number = {22},
    pages = {2961-2967},
    year = {2011},
    issn = {1680-5593},
    doi = {javaa.2011.2961.2967},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.2961.2967},
    author = {N.,M.H.,H.,R.,N.,A. and},
    keywords = {Infectious bronchitis virus,S1 gene,phylogenetic analysis,replication,foreign,Iran},
    abstract = {Ten isolates of Infectious Bronchitis Virus (IBV) were detected from the trachea and lung of IB suspected chickens from 1998-2008 in Iran by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). For further analysis, the entire <I>S1</I> gene of the IBV isolates amplified by other RT-PCR. Phylogenetic analysis based on both <I>S1</I> gene nucleotide and amino acid deduced sequences showed that isolates of Iran were classified into two genotypes Massachusetts (3 isolates) and 4/91 (7 isolates). Three isolates of Massachusetts serotype had very strong genetic relatedness to Massachusetts-type vaccines (particularly H120) indicating the probability of the detection and isolation of vaccine strains. The present study demonstrates that 4/91 type has been circulating in the country since 1998. Comparison of 4/91 isolates of Iran and almost all foreign isolates of 4/91 showed that Iranian 4/91 isolates (except for IR/491/08) had maximum average nucleotide and amino acid identities with French isolate FR-94047-94, supporting this probability that 4/91 isolates of Iran originated from France. The isolate IR/491/08 recovered from flock vaccinated with 4/91 vaccine strain and had high sequence similarity of the <I>S1</I> gene with 4/91 vaccine strain. Therefore, it is likely that this isolate was re-isolation vaccine strain. The results also revealed that the application of Massachusetts vaccines may not contain the replication of the 4/91 type.}
    }