TY - JOUR
T1 - Molecular Analysis of Infectious Bronchitis Viruses Isolated in Iran from 1998-2008
AU - Ghahremani, N. AU - Bozorgmehri Fard, M.H. AU - Shoushtari, H. AU - Momayez, R. AU - Sheikhi, N. AU - Khoshzahmat, A. AU - Eshratabadi, F.
JO - Journal of Animal and Veterinary Advances
VL - 10
IS - 22
SP - 2961
EP - 2967
PY - 2011
DA - 2001/08/19
SN - 1680-5593
DO - javaa.2011.2961.2967
UR - https://makhillpublications.co/view-article.php?doi=javaa.2011.2961.2967
KW - Infectious bronchitis virus
KW -S1 gene
KW -phylogenetic analysis
KW -replication
KW -foreign
KW -Iran
AB - Ten isolates of Infectious Bronchitis Virus (IBV) were detected from the trachea and lung of IB suspected chickens from 1998-2008 in Iran by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). For further analysis, the entire S1 gene of the IBV isolates amplified by other RT-PCR. Phylogenetic analysis based on both S1 gene nucleotide and amino acid deduced sequences showed that isolates of Iran were classified into two genotypes Massachusetts (3 isolates) and 4/91 (7 isolates). Three isolates of Massachusetts serotype had very strong genetic relatedness to Massachusetts-type vaccines (particularly H120) indicating the probability of the detection and isolation of vaccine strains. The present study demonstrates that 4/91 type has been circulating in the country since 1998. Comparison of 4/91 isolates of Iran and almost all foreign isolates of 4/91 showed that Iranian 4/91 isolates (except for IR/491/08) had maximum average nucleotide and amino acid identities with French isolate FR-94047-94, supporting this probability that 4/91 isolates of Iran originated from France. The isolate IR/491/08 recovered from flock vaccinated with 4/91 vaccine strain and had high sequence similarity of the S1 gene with 4/91 vaccine strain. Therefore, it is likely that this isolate was re-isolation vaccine strain. The results also revealed that the application of Massachusetts vaccines may not contain the replication of the 4/91 type.
ER -