Agricultural Journal
ISSN: Online 1994-4616ISSN: Print 1816-9155
Abstract
Two Iraqi varieties of wheat seeds (Alfares and Tamoz-2) were induced to form callus using growth regulators for the regeneration of plantlet from callus induction. The growth regulators used are 2,4-D 0.0, 1.5, 2.0, 2.5 and 3.0 mgL-1 and Kinetin 0.0, 0.3, 0.6, 9.0 and 1.2 mgL-1 to the induction of callus. IAA 0.0, 0.5, 1.0, 1.5 and 2.0 mgL-1 and BAP 0.0, 0.1, 0.2, 0.3 and 0.4 mgL-1 to regeneration of plantlet from callus. IBA 0.0, 2.0, 4.0, 6.0 and 8.0 mgL-1 to reform root. Results showed significant differences between wheat varieties as recorded the highest average of wet and dry weights of callus was reached 113.2 and 9.9 mg, respectively at 2.5 and 0.9 mg of 2,4-D and Kinetin, respectively for the variety (Alfares) while the lowest average of wet and dry weights of callus was reached 100.5 and 9.3 mg respectively at 2.5 and 0.9 mg of 2, 4-D and Kinetin respectively for the variety Tamoz-2. Results showed that the highest percentage of plantlet regenerationn from callus 13.8 % at 1.5 and 0.3 mg of IAA and BAP, respectively for the variety (Alfares) while the lowest percentage of plantlet regeneration from callus 0.0% at 0.0and 0.0 mg of IAA and BAP, respectively for the varieties (Alfares and Tamoz-2). Results showed that the highest and lowest percentage of roots reformation were 6.8 and 0.0% of the variety (Alfares and Tamoz-2), respectively at 6.0 and 0.0 mg, respectively from IBA.
How to cite this article:
Saja J.S. Baday. In Vitro Study the Callus Induction of Two Varieties of Wheat Seeds by
Plant Growth Regulators.
DOI: https://doi.org/10.36478/aj.2018.67.71
URL: https://www.makhillpublications.co/view-article/1816-9155/aj.2018.67.71