@article{MAKHILLAJ201813320668,
    title = {<i>In Vitro</i> Study the Callus Induction of Two Varieties of Wheat Seeds by
Plant Growth Regulators},
    journal = {Agricultural Journal},
    volume = {13},
    number = {3},
    pages = {67-71},
    year = {2018},
    issn = {1816-9155},
    doi = {aj.2018.67.71},
    url = {https://makhillpublications.co/view-article.php?issn=1816-9155&doi=aj.2018.67.71},
    author = {Saja},
    keywords = {2,4-D,Kinetin,IAA,BAP,IBA,wheat,callus},
    abstract = {Two Iraqi varieties of wheat seeds (Alfares and Tamoz-2) were induced to form callus using growth
regulators for the regeneration of plantlet from callus induction. The growth regulators used are 2,4-D 0.0, 1.5,
2.0, 2.5 and 3.0 mgL<sup>-1</sup> and Kinetin 0.0, 0.3, 0.6, 9.0 and 1.2 mgL<sup>-1</sup> to the induction of callus. IAA 0.0, 0.5, 1.0, 1.5
and 2.0 mgL<sup>-1</sup> and BAP 0.0, 0.1, 0.2, 0.3 and 0.4 mgL<sup>-1</sup> to regeneration of plantlet from callus. IBA 0.0, 2.0, 4.0, 6.0
and 8.0 mgL<sup>-1</sup> to reform root. Results showed significant differences between wheat varieties as recorded the
highest average of wet and dry weights of callus was reached 113.2 and 9.9 mg, respectively at 2.5 and 0.9 mg
of 2,4-D and Kinetin, respectively for the variety (Alfares) while the lowest average of wet and dry weights of
callus was reached 100.5 and 9.3 mg respectively at 2.5 and 0.9 mg of 2, 4-D and Kinetin respectively for the
variety Tamoz-2. Results showed that the highest percentage of plantlet regenerationn from callus 13.8 % at
1.5 and 0.3 mg of IAA and BAP, respectively for the variety (Alfares) while the lowest percentage of plantlet
regeneration from callus 0.0% at 0.0and 0.0 mg of IAA and BAP, respectively for the varieties (Alfares and
Tamoz-2). Results showed that the highest and lowest percentage of roots reformation were 6.8 and 0.0% of
the variety (Alfares and Tamoz-2), respectively at 6.0 and 0.0 mg, respectively from IBA.}
    }