Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
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Investigation of Phenotypic and Genotypic Characteristics of Isolation of Strains Vagococcus salmoninarum Cage Business Rainbow Trouts (Oncorhynchus mykiss) of Kahramanmaras Province (Turkey)
M. Őzcan and Y. Baris
Page: 124-131 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
In this study, it was included to identify and investigate phenotypic and genotypic charecteristics the isolation of Vagococcus salmoninarum in rainbow trout farms the cage of Kahramanmaras Province (Turkey). For this purpose, farms twenty different trout between July 2014 and June 2013 were taken in samples the gut and kidney, spleen, liver, eye rainbow trout weighing 250-300 g. For isolation of Vagococcus salmoninarum; tryptic soy agar, nutrient broth and brain heart infusion agars were used. The pure strains obtained from these samples from trout farms were evaluated phenotypic characteristics applied with Biolog system (The biolog GENIII micro plate) connection and biochemical tests. Antibiogram sensitivity test was also applied, according to disk diffusion method. General primer were used to confirm 53 Vagococcus salmoninarum with PCR after determined, according to bacteria the phenotype and genotype characteristics. Subsequently, these strains were confirmed as Vagococcus salmoninarum with DNA. The RFLP (Restriction Fragment Length Polymorphism) the strain of confirmed PCR (Polymerase Chain Reaction) were measured of features gene by the technique. Vagococcus salmoninarum was made from sequence analysis one of the resulting sample and the analysis result was interpreted from data obtained. Sub-typing Vagococcus salmoninarum with RFLP technique was performed successfully.
How to cite this article:
M. Őzcan and Y. Baris. Investigation of Phenotypic and Genotypic Characteristics of Isolation of
Strains Vagococcus salmoninarum Cage Business Rainbow Trouts
(Oncorhynchus mykiss) of Kahramanmaras Province (Turkey).
DOI: https://doi.org/10.36478/javaa.2019.124.131
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2019.124.131