M.  Őzcan, Y.  Baris, 
    Investigation of Phenotypic and Genotypic Characteristics of Isolation of
Strains Vagococcus salmoninarum Cage Business Rainbow Trouts
(<i>Oncorhynchus mykiss</i>) of Kahramanmaras Province (Turkey),
    Journal of Animal and Veterinary Advances,
    Volume 18,Issue 4,
    2019,
    Pages 124-131,
    ISSN 1680-5593,
    javaa.2019.124.131,
    (https://makhillpublications.co/view-article.php?doi=javaa.2019.124.131)
    Abstract: In this study, it was included to identify and investigate phenotypic and genotypic charecteristics
the isolation of <i>Vagococcus salmoninarum</i> in rainbow trout farms the cage of Kahramanmaras Province
(Turkey). For this purpose, farms twenty different trout between July 2014 and June 2013 were taken in samples
the gut and kidney, spleen, liver, eye rainbow trout weighing 250-300 g. For isolation of Vagococcus
salmoninarum; tryptic soy agar, nutrient broth and brain heart infusion agars were used. The pure strains
obtained from these samples from trout farms were evaluated phenotypic characteristics applied with Biolog
system (The biolog GENIII micro plate) connection and biochemical tests. Antibiogram sensitivity test was also
applied, according to disk diffusion method. General primer were used to confirm 53 <i>Vagococcus salmoninarum</i>
with PCR after determined, according to bacteria the phenotype and genotype characteristics. Subsequently,
these strains were confirmed as <i>Vagococcus salmoninarum</i> with DNA. The RFLP (Restriction Fragment Length
Polymorphism) the strain of confirmed PCR (Polymerase Chain Reaction) were measured of features gene by
the technique. <i>Vagococcus salmoninarum</i> was made from sequence analysis one of the resulting sample and
the analysis result was interpreted from data obtained. Sub-typing <i>Vagococcus salmoninarum</i> with RFLP
technique was performed successfully.
    Keywords: Oncorhynchus mykiss;phenotypic;genotypic;Vagococcus salmoninarum;Biolog GEN III;PCR;RFLP;sequence