Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
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Abstract
To clone the IL-10 promoter of rabbit and analyse its sequence, this study amplified the 5 flanking region of rabbit IL-10, compared the genomes and used bioinformatics software to make a forecast. Then, the researchers comfirmed partial promoter sequences and analysed some transcription factors of the IL-10 gene promoter. The study showed that rabbit and human IL-10 gene promoter had some similarities which all cotained GAGA, CAAT and TATA. The transcriptional start site started from -80 bp of translation initiation codon (ATG) upstream region. The promoter also had some transcription factors such as AP-1, Myogenin, c-Ets-1, C/EBP alpha and so on. Results of this study predicted partial sequences, transcriptional start site and some transcriptional regulating factors binding sites of IL-10 gene promoter which would lay the foundation of lucubrating the expression and regulation of rabbit IL-10 gene.
How to cite this article:
Wu Xin-Sheng, He Meng-Jie, Zhang Lei, Feng Kai, Wang Xiao-Ming, Pan Yu-Lai, Wan Xiao-Ying and Mao Liu-Liu. Cloning and Sequence Analysis of Rabbit Interleukin-10 (IL-10) Gene Promoter.
DOI: https://doi.org/10.36478/javaa.2012.323.326
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2012.323.326