Wu  Xin-Sheng, He  Meng-Jie, Zhang  Lei, Feng  Kai, Wang  Xiao-Ming, Pan  Yu-Lai, Wan  Xiao-Ying, Mao  Liu-Liu, 
    Cloning and Sequence Analysis of Rabbit Interleukin-10 (<I>IL-10</I>) Gene Promoter,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 3,
    2012,
    Pages 323-326,
    ISSN 1680-5593,
    javaa.2012.323.326,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.323.326)
    Abstract: To clone the IL-10 promoter of rabbit and analyse its sequence, this study amplified the 5 flanking region of rabbit IL-10, compared the genomes and used bioinformatics software to make a forecast. Then, the researchers comfirmed partial promoter sequences and analysed some transcription factors of the <I>IL-10</I> gene promoter. The study showed that rabbit and human <I>IL-10</I> gene promoter had some similarities which all cotained GAGA, CAAT and TATA. The transcriptional start site started from -80 bp of translation initiation codon (ATG) upstream region. The promoter also had some transcription factors such as AP-1, Myogenin, c-Ets-1, C/EBP alpha and so on. Results of this study predicted partial sequences, transcriptional start site and some transcriptional regulating factors binding sites of <I>IL-10</I> gene promoter which would lay the foundation of lucubrating the expression and regulation of rabbit <I>IL-10</I> gene.
    Keywords: bioinformatics;transcription factor;promoter;IL-10;gene;China