Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
Abstract
The muscular tissue of breast was dissected from 8 weeks old Jilin White goose in the present study. The big fragment PCR Method was used to amplify double-strand cDNA based on the SMART techniques for construction of a full-length cDNA library. After digestion with restriction endonuclease Sfi |, a modified vector of pBluescript II SK-plasmid with the adaptors containing Sfi |A and Sfi |B sites was used to recombine with the cDNA products amplified. The recombinants were cloned by transformation into competent Escherichia coli DH2α. A plasmid cDNA library with goose muscle was constructed. The results showed that the titer of the cDNA library was 1.01x106 pfu mL-1 and the percentage of recombinant clones was 97%. The length of most cDNA inserted was between 0.25 and 1.6 kb identified by gel electrophoresis after cDNA PCR amplification. The unigene ratio was 66.7% and the percentage of complete cDNA sequences was 80% by estimating from the 24 clones sequenced randomly. It is helpful to study muscle development of goose at molecular level in the future.
How to cite this article:
Wei Wu, Lu Chen, Haiyang Zhu, Yujian Sui, Yingying Fu, Zhe Hao, Jingtao Hu, Tongao Yang and Yongfeng Sun. Construction of Full-Length Goose Muscle cDNA Library.
DOI: https://doi.org/10.36478/javaa.2012.2106.2109
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2012.2106.2109