Wei  Wu, Lu  Chen, Haiyang  Zhu, Yujian  Sui, Yingying  Fu, Zhe  Hao, Jingtao  Hu, Tongao  Yang, Yongfeng  Sun, 
    Construction of Full-Length Goose Muscle cDNA Library,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 12,
    2012,
    Pages 2106-2109,
    ISSN 1680-5593,
    javaa.2012.2106.2109,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.2106.2109)
    Abstract: The muscular tissue of breast was dissected from 8 weeks old Jilin White goose in the present study. The big fragment PCR Method was used to amplify double-strand cDNA based on the SMART techniques for construction of a full-length cDNA library. After digestion with restriction endonuclease Sfi &#124;, a modified vector of pBluescript II SK-plasmid with the adaptors containing Sfi &#124;A and Sfi &#124;B sites was used to recombine with the cDNA products amplified. The recombinants were cloned by transformation into competent <I>Escherichia coli</I> DH2&#945;. A plasmid cDNA library with goose muscle was constructed. The results showed that the titer of the cDNA library was 1.01x10<SUP>6</SUP> pfu mL<SUP>-1</SUP> and the percentage of recombinant clones was 97%. The length of most cDNA inserted was between 0.25 and 1.6 kb identified by gel electrophoresis after cDNA PCR amplification. The unigene ratio was 66.7% and the percentage of complete cDNA sequences was 80% by estimating from the 24 clones sequenced randomly. It is helpful to study muscle development of goose at molecular level in the future.
    Keywords: China;PCR amplification;SMART technique;cDNA library;Goose