Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
Abstract
The fimbrial gene of Pasteurella multocida B:2, isolated from cattle with haemorrhagic septicaemia was amplified, cloned, sequenced and expressed in a vector. Cloning of the fimbrial gene revealed a 435 bp band while sequencing revealed similar nucleotide sequencing to the PtfA gene of P. multocida A:3 isolated from chicken, except the five nucleotide changes at residues 171, 282, 363, 387 and 414. The fimbrial gene was found to encode a deduced protein of 144 amino acids. The expressed fimbrial protein was detected from 4 h post-induction with a molecular weight of 18-kDa.
How to cite this article:
A. Ernie , M. Zamri-Saad , Z. Zunita , A. R. Omar , B. Siti Khairani and M. Y. Sabri . Sequencing and Expression of Fimbrial Gene of Pasteurella multocida B:2.
DOI: https://doi.org/10.36478/javaa.2005.254.259
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2005.254.259