@article{MAKHILLJAVA200542213, title = {Sequencing and Expression of Fimbrial Gene of Pasteurella multocida B:2}, journal = {Journal of Animal and Veterinary Advances}, volume = {4}, number = {2}, pages = {254-259}, year = {2005}, issn = {1680-5593}, doi = {javaa.2005.254.259}, url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2005.254.259}, author = {A. Ernie,M. Zamri-Saad,Z. Zunita,A. R. Omar,B. Siti Khairani and}, keywords = {}, abstract = {The fimbrial gene of Pasteurella multocida B:2, isolated from cattle with haemorrhagic septicaemia was amplified, cloned, sequenced and expressed in a vector. Cloning of the fimbrial gene revealed a 435 bp band while sequencing revealed similar nucleotide sequencing to the PtfA gene of P. multocida A:3 isolated from chicken, except the five nucleotide changes at residues 171, 282, 363, 387 and 414. The fimbrial gene was found to encode a deduced protein of 144 amino acids. The expressed fimbrial protein was detected from 4 h post-induction with a molecular weight of 18-kDa.} }