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Journal of Animal and Veterinary Advances

ISSN: Online 1993-601X
ISSN: Print 1680-5593
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Conserved Gene Structure and Function of Interleukin-10 in Teleost Fish

Bo-Hye Nam, Ji-Young Moon, Eun-Hee Park, Young-Ok Kim, Dong-Gyun Kim, Hee Jeong Kong, Woo-Jin Kim, Hyungtaek Jung, Young Ju Jee, Sang-Jun Lee and Cheul min An
Page: 774-782 | Received 21 Sep 2022, Published online: 21 Sep 2022

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https://doi.org/10.36478/javaa.2014.774.782

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Abstract

Interleukin-10 (IL-10) is an important immunoregulatory cytokine produced by various types of cells. Researchers describe here the isolation and characterization of olive flounder IL-10 (ofIL-10) cDNA and genomic organization. The ofIL-10 gene encodes a 187 amino acid protein and is composed of a five exon/four intron structure, similar to other known IL-10 genes. The ofIL-10 promoter sequence analysis shows a high level of homology in putative binding sites for transcription factors which are sufficient for transcriptional regulation ofIL-10. Important structural residues are maintained in the ofIL-10 protein including the four cysteines responsible for the two intra-chain disulfide bridges reported for human IL-10 and two extra cysteine residues that exist only in fish species. The phylogenetic analysis clustered ofIL-10 with other fish IL-10s and apart from mammalian IL-10 molecules. Quantitative real-time Polymerase Chain Reaction (PCR) analysis demonstrated ubiquitous ofIL-10 gene expression in the 13 tissues examined. Additionally, the induction of ofIL-10 gene expression was observed in the kidney tissue from olive flounder infected with bacteria (Edawardsiella tarda) or virus (Viral Hemorrhagic Septicemia Virus; VHSV). These data indicate that IL-10 is an important immune regulator that is conserved strictly genomic organization and function during the evolution of vertebrate immunity.

How to cite this article:

Bo-Hye Nam, Ji-Young Moon, Eun-Hee Park, Young-Ok Kim, Dong-Gyun Kim, Hee Jeong Kong, Woo-Jin Kim, Hyungtaek Jung, Young Ju Jee, Sang-Jun Lee and Cheul min An. Conserved Gene Structure and Function of Interleukin-10 in Teleost Fish.
DOI: https://doi.org/10.36478/javaa.2014.774.782
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2014.774.782