Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
89
Views
1
Downloads
Evaluation of the Suitability of Five Housekeeping Genes as Internal Control for Quantitative Real-Time RT-PCR Assays in Chickens Acutely Infected with IBV M41 Strain
Wen-Qiao Fan, Hong-Ning Wang, Yi Zhang and An-Yun Zhang
Page: 3064-3067 | Received 21 Sep 2022, Published online: 21 Sep 2022
Full Text Reference XML File PDF File
Abstract
Avian Infectious Bronchitis (IB) is an acute and highly contagious respiratory disease in chickens. To detect the virus in chickens, housekeeping genes were detected by quantitative real-time PCR for the normalization. The aim of study was to identify the most stable housekeeping gene (s) in IBV infection. Five housekeeping genes including 18S rRNA, β-actin, Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), Glucose-6-Phosphate Dehydrogenase (G6PDH) and Ubiquitin (UB) were investigated in nine tissues: trachea, thymus, liver, spleen, lungs, kidney, pancreas, proventriculus and bursa of Fabricius. The geNorm and Normfinder Software were used to calculate the stability of the candidate genes.
How to cite this article:
Wen-Qiao Fan, Hong-Ning Wang, Yi Zhang and An-Yun Zhang. Evaluation of the Suitability of Five Housekeeping Genes as Internal Control for Quantitative Real-Time RT-PCR Assays in Chickens Acutely Infected with IBV M41 Strain.
DOI: https://doi.org/10.36478/javaa.2012.3064.3067
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2012.3064.3067