@article{MAKHILLJAVA201211173681,
title = {Evaluation of the Suitability of Five Housekeeping Genes as Internal Control for Quantitative Real-Time RT-PCR Assays in Chickens Acutely Infected with IBV M41 Strain},
journal = {Journal of Animal and Veterinary Advances},
volume = {11},
number = {17},
pages = {3064-3067},
year = {2012},
issn = {1680-5593},
doi = {javaa.2012.3064.3067},
url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.3064.3067},
author = {Hong-Ning,Wen-Qiao,Yi and},
keywords = {Avian infectious bronchitis virus,housekeeping genes,quantitative real-time reverse transcription-polymerase chain reaction,suitability,China},
abstract = {Avian Infectious Bronchitis (IB) is an acute and highly contagious
respiratory disease in chickens. To detect the virus in chickens, housekeeping
genes were detected by quantitative real-time PCR for the normalization. The
aim of study was to identify the most stable housekeeping gene (s) in IBV infection.
Five housekeeping genes including 18S rRNA, β-actin, Glyceraldehyde-3-Phosphate
Dehydrogenase (GAPDH), Glucose-6-Phosphate Dehydrogenase (G6PDH)
and Ubiquitin (UB) were investigated in nine tissues: trachea, thymus,
liver, spleen, lungs, kidney, pancreas, proventriculus and bursa of Fabricius.
The geNorm and Normfinder Software were used to calculate the stability of the
candidate genes.}
}