Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
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An Improved Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Sensitive and Specific Detection of Infectious Bronchitis Virus
Hongbin Luo, Daozhong Zhu, Chunyi Xue, Jianping Qin, Feng Chen and Yongchang Cao
Page: 2398-2402 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
A highly conserved region of 1a gene of Infectious Bronchitis Virus (IBV) was chosen to design the Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) degenerate primers. The developed RT-LAMP assay is a highly sensitive, specific, rapid method to detect IBV in allantoid and tissue. Optimal temperature was around 62°C and duration was 45 min. Sensitivity analysis showed that RT-LAMP assay was 10 fold more sensitive than RT-PCR. Specificity analysis showed that 3 IBV strains omitted by the previous reported RT-LAMP assay could be detected by the improved RT-LAMP assay. In field trials, the improved RT-LAMP assay obtained 98.4% sensitivity in 65 clinical samples while 95.4% of RT-PCR and 93.8% of the previous reported RT-LAMP which indicates it is a powerful tool in the practical application.
How to cite this article:
Hongbin Luo, Daozhong Zhu, Chunyi Xue, Jianping Qin, Feng Chen and Yongchang Cao. An Improved Reverse Transcription Loop-Mediated Isothermal Amplification Assay
for Sensitive and Specific Detection of Infectious Bronchitis Virus.
DOI: https://doi.org/10.36478/javaa.2012.2398.2402
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2012.2398.2402