Yongchang  Cao, Hongbin  Luo, Daozhong  Zhu, Chunyi  Xue, Jianping  Qin, Feng  Chen, 
    An Improved Reverse Transcription Loop-Mediated Isothermal Amplification Assay 
  for Sensitive and Specific Detection of Infectious Bronchitis Virus,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 14,
    2012,
    Pages 2398-2402,
    ISSN 1680-5593,
    javaa.2012.2398.2402,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.2398.2402)
    Abstract: A highly conserved region of <I>1a </I>gene of Infectious Bronchitis Virus (IBV) was chosen to design the Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) degenerate primers. The developed RT-LAMP assay is a highly sensitive, specific, rapid method to detect IBV in allantoid and tissue. Optimal temperature was around 62&deg;C and duration was 45 min. Sensitivity analysis showed that RT-LAMP assay was 10 fold more sensitive than RT-PCR. Specificity analysis showed that 3 IBV strains omitted by the previous reported RT-LAMP assay could be detected by the improved RT-LAMP assay. In field trials, the improved RT-LAMP assay obtained 98.4% sensitivity in 65 clinical samples while 95.4% of RT-PCR and 93.8% of the previous reported RT-LAMP which indicates it is a powerful tool in the practical application.
    Keywords: Infectious bronchitis virus;loop-mediated isothermal amplification;IBV strains;RT-LAMP