Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
Abstract
The viability, Purity and count of monocytes isolated from peripheral blood of camels were assessed after the isolation of these cells on 9, 10 and 12% concentrations of Ficoll/Hypaque. The results obtained showed that 97% viable cells were obtained using the three concentrations of the Ficoll/Hypaque. For purity, 12% Ficoll concentration separated cells were contaminated with others blood cells, while 9 and 10% separated cells were pure. When cells count was applied for the pure isolated cells, the cells isolated on 10% Ficoll/Hypaque concentration showed the best highest count compared to 9% with p = 0.003. Cells isolated using 10% Ficoll/Hypaque well proliferated when stimulated with Phytohaemaggglutnin (PHA) mitogen.
How to cite this article:
Layla Mohamed and M.M. Mukhtar . Optimum Ficoll/Hypaque Gradient for Efficient Isolation of Peripheral Blood Monocytes from Camels.
DOI: https://doi.org/10.36478/javaa.2007.152.154
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2007.152.154