@article{MAKHILLJAVA200762246, title = {Optimum Ficoll/Hypaque Gradient for Efficient Isolation of Peripheral Blood Monocytes from Camels}, journal = {Journal of Animal and Veterinary Advances}, volume = {6}, number = {2}, pages = {152-154}, year = {2007}, issn = {1680-5593}, doi = {javaa.2007.152.154}, url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2007.152.154}, author = {Layla Mohamed and}, keywords = {Ficoll/Hypaque,viable cells,peripheral blood,PHA,monocytes}, abstract = {The viability, Purity and count of monocytes isolated from peripheral blood of camels were assessed after the isolation of these cells on 9, 10 and 12% concentrations of Ficoll/Hypaque. The results obtained showed that 97% viable cells were obtained using the three concentrations of the Ficoll/Hypaque. For purity, 12% Ficoll concentration separated cells were contaminated with others blood cells, while 9 and 10% separated cells were pure. When cells count was applied for the pure isolated cells, the cells isolated on 10% Ficoll/Hypaque concentration showed the best highest count compared to 9% with p = 0.003. Cells isolated using 10% Ficoll/Hypaque well proliferated when stimulated with Phytohaemaggglutnin (PHA) mitogen.} }