Abstract

The nematocidal activity of hydroalcoholic extract obtained from the bulbs of A. hirtifolium was investigated. Mortality average of Rhabditis sp. filariform larvae for concentrations 1.250, 0.312 and 0.075 mg mL^-1 was 52.3, 47.6 and 35%, respectively. The extract showed anthelmintic activity against Rhabditis sp.

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INTRODUCTION

Nematodes of the family Rhabditidae are abundant in soil and water. The nematodes of this family have free living and parasitic forms in their life cycles (Abolafia and Pena-Santiago, 2007). In this study, we used Rhabditis sp. as model for anthelmintic research at first time. Persian shallot or Allium hirtifolium is a species of the family Liliaceae, a native plant in Iran (Harris et al., 2001; Taran et al., 2006).Traditional medicine uses genus Allium plants such as A. sativum, A. schoenoprasum, A. cepa for treatment of infectous diseases such as bacterial, fungal, viral, protozoal and helminthic diseases (Harris et al., 2001; Taran et al., 2006). The aim of this study is to present anthelmintic activity of A. hirtifolium hydroalcoholic extract against Rhabditis sp.

MATERIALS AND METHODS

Plant materials: For preparation of A. hirtifolium hydroalcoholic extract, A. hirtifolium bulbs were collected from Khansar, Iran. Then, the small slices of A. hirtifolium bulbs were macerated in ethanol/water (50:50) for one month at 10°C and the filtrated extract air-dried in room temperature.

Nematode: For evaluation of nematocide activity of hydroalcoholic extract of A. hirtifolium, filariform larvae of Rhabditis sp. Prepared in Helminthology laboratory, Department of parasitology, Tehran University of medical sciences, Tehran, Iran.

Nematocide evaluation of hydroalcoholic extract obtained from A. hertifolium: Filariform larvae of Rhabditis sp. were collected from agar plate. Then, approximately 50 larvae added to each concentration of hydroalcoholic extract of A. hertifolium. nematocide activity for each concentration was tested three times. The larvae viability was determined by microscope.

RESULTS AND DISCUSSION

In this study, mortality average of Rhabditis sp. filariform larvae for concentrations 1.250, 0.312 and 0.075 mg mL^-1 of hydroalcoholic extract was 52.3, 47.6 and 35%, respectively. A. hirtifolium killed larvae of Rhabditis sp. at high concentrations. Therefore, this plant has some nematocide components. The plants of genus Allium have parasitic agents such as allicin, ajoen and other organosulfide (Harris et al., 2001; Urbina et al., 1993). Parasitic cells are more sensitive than host cells to allicin and allicin-derived componenets(Urbina et al., 1993). The extracts of some species of genus Allium reduce growth of organisms by inhibition the synthesis of macromolecules and decreasing the oxygen uptake (Szymona, 1952; Adetumbi et al., 1986; Ghannoum, 1988; Harris et al., 2001).

CONCLUSION

In this study, hydroalcoholic extract of A. hirtifolium exhibits anthelmintic activity against filariform larvae of Rhabditis sp.

ACKNOWLEDGEMENT

Researchers thank Dr. A. Soltanpoor for his help to provide the Persian shallot extract.

How to cite this article:

Mojtaba Taran and Mohamad Izaddoost. Nematocidal Activity of Allium hirtifolium (Persian Shallot) Against Rhabditis sp..
DOI: https://doi.org/10.36478/vr.2010.27.28
URL: https://www.makhillpublications.co/view-article/1993-5412/vr.2010.27.28