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Veterinary Research

ISSN: Online 1994-4659
ISSN: Print 1993-5412
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In vitro Antibacterial Activity tests of Cinnamon Bark Essential Oil and Cinnamaldehyde Compounds Against Mastitis Causal Agents Isolated from Milk Shed Area of Debrebirhan Zone, North Shoa, Ethiopia

C. Yeshimebet , G. Getachew , W. Solomon , A. Derib , A. Tadios , D. Chekol , D. Aschalew , A. Ermias , M. Yomiyu and O. Tamiru
Page: 1-7 | Received 27 May 2023, Accepted 26 Apr 2022, Published online: 13 May 2022

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https://doi.org/10.36478/10.59218/makvr.2022.1.7

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Abstract

The knowledge of plants as therapeutic agents goes back to the beginning of time. Ancient texts provide valuable clues of plants being used as potentially antibacterial compounds. The plant was selected due to its application as traditional medicinal plant. The potent antimicrobial activities of essential oil extracts of Cinnamon were screened for in vitro activity against Staphylococcus aureus, using standard Agar Disc-Diffusion assay. Streptomycin, penicillin, Nalidixc acid, Cephalotin, oxytetracycline and Cephoperazone were used as a reference standard for the comparative evaluation resulting that the Staphylococcus aureus found resistance to the tested antibiotics discs. The antimicrobial activity of Cinnamon (CINA) bark essential oil for CINA10 µL found intermediate whereas CINA15, for 20 and 30 µL sensitivity disks, exhibited high antimicrobial effect against Staphylococcus aureus with the inhibition zone of 15.33±0.58, 20.00±1.00 and 27.33±5.03, respectively followed by the dilution of Cinnamon and Acetone (CINAacet 30, 50 and 70%) using 30 µL disks, exhibited high zone of inhibition 15.00±3.00, 17.66±0.58 and 22.33±5.85. This confirms that Cinnamon contains substances like tannin soluble, astringent complex phenolic substance and saponin with antimicrobial properties. Then again Cinnamon main component (Cinnamaldehyde) were tested against Escherichia coli (ATCC 2592), Streptococcus agalactiae (ATCC 12380), Staphylococcus aureus (ATCC 25923) obtained from Ethiopian Public Health Institute (EPHI) and also Staphylococcus aureus and Escherichia coli isolated from smallholder farms mastitic cows using different microbiological methods, Agar Well Diffusion, Agar Disk Diffusion, Minimum Inhibitory Concentration and Minimum Bacterial Concentration and showed high antimicrobial activities.

How to cite this article:

C. Yeshimebet , G. Getachew , W. Solomon , A. Derib , A. Tadios , D. Chekol , D. Aschalew , A. Ermias , M. Yomiyu and O. Tamiru . In vitro Antibacterial Activity tests of Cinnamon Bark Essential Oil and Cinnamaldehyde Compounds Against Mastitis Causal Agents Isolated from Milk Shed Area of Debrebirhan Zone, North Shoa, Ethiopia.
DOI: https://doi.org/10.36478/10.59218/makvr.2022.1.7
URL: https://www.makhillpublications.co/view-article/1993-5412/10.59218/makvr.2022.1.7