Research Journal of Dairy Sciences
ISSN: OnlineISSN: Print 1993-5277
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Early Detection of Aspergillus fumgitus in a High Risk Group of Dairy Cattle using Nested and Real-Time Quantitative PCR
Liangcheng Chen, Tao Jiang, Xiao Li, Qi Wang, Yu Li, Kevin Marley and Yongqiang Wang
Page: 34-40 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
Early detection of Aspergillus fumigatus (A. fumigatus) may facilitate early diagnosis and prevention of Invasive Aspergillosis (IA) in dairy cows. This study used a nested PCR and real-time quantitative PCR method to measure A. fumigatus in feed, tissue and blood samples collected from domestic dairy farms in China. In total, 396 feed samples from across China including corn, soybean meal, cotton seed meal, wheat mill run, distiller’s dried grains, corn and grass silage and Total Mixed Ration (TMR) were tested. Of these, 95% contained detectable levels of A. fumigatus. Blood and tissue samples from the GI tract of 7 unhealthy cows all tested positive for A. fumigatus. The analytical sensitivity of the PCR method was<1 fg/assay.
How to cite this article:
Liangcheng Chen, Tao Jiang, Xiao Li, Qi Wang, Yu Li, Kevin Marley and Yongqiang Wang. Early Detection of Aspergillus fumgitus in a High Risk Group of Dairy Cattle using Nested and
Real-Time Quantitative PCR.
DOI: https://doi.org/10.36478/rjdsci.2014.34.40
URL: https://www.makhillpublications.co/view-article/1993-5277/rjdsci.2014.34.40