Research Journal of Animal Sciences
ISSN: Online 1994-4640ISSN: Print 1993-5269
Abstract
Secondary structure prediction is vital for determining protein’s structure and functions, whereas phylogenetic analysis provides an understanding of how species evolve due to genetic alterations. Since the IGF-1 gene is a major participant in the developmental and reproductive abilities of poultry and other animals, its potential as a molecular marker was thus assessed. Sixty local chicken strains-normal feathered, frizzle feathered and naked neck-comprising twenty from each strain, from which fifteenfive was sampled per strain for blood collection and DNA extraction) were involved in the work. Jena Bioscience Gmbh preparation kit was used in extracting DNA, while the Shine Gene Primers given by: GTCGGGCTACTTGAGTTACTAC-Forward. TTGCGCAGGCTCTATCT GCTCReverse, was used to identify genomic DNA for sequencing of the gene (IGF-1). About 2% agarose gel was used to assess the DNA purity. Results showed variation in the secondary structure of IGF-1 gene in these strains with the naked neck having a higher percentage of alpha helix, beta turn and random coil than the others and the mingling of the strains with other Gallus species in the phylogenetic tree. This is an indication of its ability to withstand mutation which makes it stable. Thus, making IGF-1 a marker of interest in the genomic selection of chicken for development and improvement.
How to cite this article:
L.J. Isaac, B. Okon, L.A. Ibom and A. Dauda. Secondary Structures and Phylogenetic Relationship in the IGF 1 Gene Sequence of Three Local Chicken Strains.
DOI: https://doi.org/10.36478/makrjans.2025.1.1.6
URL: https://www.makhillpublications.co/view-article/1993-5269/makrjans.2025.1.1.6