files/journal/2022-09-02_14-24-19-000000_229.png

Journal of Fisheries International

ISSN: Online 1993-6025
ISSN: Print 1817-3381
About US
Editors
Issues
Pages
Indexed in
134
Views
0
Downloads

PCR Assay for Experimental Detection of Streptococcus agalactiae Based on ScpB Gene in Rainbow Trout (Oncorhynchus mykiss)

M.A. Nematollahi, A.R. Mirvaghefi, H. Farahmand, S. Rashidi Monfared and S.A. Pour Bakhsh
Page: 75-79 | Received 21 Sep 2022, Published online: 21 Sep 2022

Download Citation
https://doi.org/10.36478/jfish.2011.75.79

Full Text Reference XML File PDF File

Abstract

Streptococcus agalactiae (Group B, GBS) is an important human and animal pathogen that causes invasive infection in fish. Present research was conducted by PCR experimental detection of S. agalactiae using scpB gene. One group of 48 fishes were challenged intramuscular (i.m.) with 0.5 mL bacterial suspension containing of 3.5x107 CFU/0.5 mL with normal saline and compared with control group that were injected by 0.5 mL sterile saline. Kidney tissue was selected for testing. Results showed that in 26 days, mortality ranged was 9.37% in challenged group. S. agalactiae was successfully detected by PCR in 42 challenged samples (87.5%).

How to cite this article:

M.A. Nematollahi, A.R. Mirvaghefi, H. Farahmand, S. Rashidi Monfared and S.A. Pour Bakhsh. PCR Assay for Experimental Detection of Streptococcus agalactiae Based on ScpB Gene in Rainbow Trout (Oncorhynchus mykiss).
DOI: https://doi.org/10.36478/jfish.2011.75.79
URL: https://www.makhillpublications.co/view-article/1817-3381/jfish.2011.75.79