Agricultural Journal
ISSN: Online 1994-4616ISSN: Print 1816-9155
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Molecular Characterization, Alternation Splicing, Expression Profile and Association Analysis with Carcass Traits of the Cattle CMYA1 Gene
Y.W. Nie, X.B. Ding, X.F. Liu, X.G. Ge, J.Y. Li, F.F. Yang, T. Guo and H. Guo
Page: 276-281 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
The Cardiomyopathy Associated protein 1 (CMYA1) is a actin binding protein which plays an important role in cardiac morphogenesis. In this study, researchers obtained the 6,272 bp mRNA sequence of the cattle CMYA1 gene, including the full coding region and encoding a protein of 1,820 amino acids. The sequence was deposited into the GenBank nucleotide database (HQ111436.1). The human (NM_194293) and mice (NM_011724) homologues have a 77.31 and 82.23% identity with the cattle CMYA1 gene, respectively. Tissue expression analysis showed that the cattle CMYA1 gene was expressed exclusively in striated muscle tissue. Moreover, one deletion variant lacking 382 bp from exon 2 was detected by RT-PCR. Two synonymous SNP rs110346467 and SNP rs110575295 in the exon 2 detected, as restriction fragment length polymorphism showed genotype frequencies among 120 crossbred cattle. Association analysis showed that a SNP rs110575295 was significantly associated with the intermuscular fat percentage between 12th and 13th ribs (p<0.05). This study will lay the groundwork for the further investigations on the detailed function of CMYA1 in cattle models.
How to cite this article:
Y.W. Nie, X.B. Ding, X.F. Liu, X.G. Ge, J.Y. Li, F.F. Yang, T. Guo and H. Guo. Molecular Characterization, Alternation Splicing, Expression Profile and Association Analysis with Carcass Traits of the Cattle CMYA1
Gene.
DOI: https://doi.org/10.36478/aj.2013.276.281
URL: https://www.makhillpublications.co/view-article/1816-9155/aj.2013.276.281