files/journal/2022-09-02_12-25-11-000000_331.png

International Journal of Tropical Medicine

ISSN: Online 1818-779X
ISSN: Print 1816-3319
About US
Editors
Issues
Pages
Indexed in
162
Views
13
Downloads

A Study on Dengue Serotyping Using Reverse Transcription PCR From Clinical Samples in a Tertiary Care Hospital

Srividya Yeruva, Nanditha Addagatla, Shumaila Abdul Rehman, Metta Madhuri, V. Sudha Rani and Mote Srinath
Page: 48-51 | Received 22 Oct 2023, Published online: 01 Nov 2023

Download Citation
https://doi.org/10.36478/10.59218/makrjms.2023.3.48.51

Full Text Reference XML File PDF File

Abstract

Dengue is one of the most common arthropod‐borne viral disease of public health importance. Dengue (DEN) virus comprises of four serotypes (DEN1, DEN2, DEN3 and DEN4). Serological assay for detection of Non‐Structural protein1 Antigen (NS1Ag), Immunoglobulins‐IGM and IGG using Enzyme‐linked immunosorbent assay (ELISA) is one of the routinely used diagnostic method. However, with the molecular methodology by Reverse Transcription Polymerase chain reaction (RT‐PCR) detection of the serotypes can be performed for epidemiological purposes. A prospective study was conducted. In Department of Clinical Microbiology, at Osmania Medical College, Hyderabad, Telangana from July 2022 to December 2022. Blood samples were collected from clinically suspected dengue patients during study period. Out of 184 Ns1 Ag positive samples, 110 were subjected to RT‐PCR. Among which 48 samples serotyping results were positive. Out of them, majority 22 (45%) belonged to DEN2, followed by DEN3 (18.75%), DEN1(12.5%). Coinfection with multiple serotypes was detected in the present study. Male preponderance (54.16%) was seen among dengue cases. As relatively few studies have been conducted for the detection of dengue serotypes by RT‐PCR. Hence this study is carried out to identify the prevalent serotypes of dengue virus in our area and their association with the clinical severity. Therefore, effective surveillance of serotypes will be useful in planning the preventive and therapeutic strategies in dengue fever.

How to cite this article:

Srividya Yeruva, Nanditha Addagatla, Shumaila Abdul Rehman, Metta Madhuri, V. Sudha Rani and Mote Srinath. A Study on Dengue Serotyping Using Reverse Transcription PCR From Clinical Samples in a Tertiary Care Hospital.
DOI: https://doi.org/10.36478/10.59218/makrjms.2023.3.48.51
URL: https://www.makhillpublications.co/view-article/1816-3319/10.59218/makrjms.2023.3.48.51