International Journal of Tropical Medicine
ISSN: Online 1818-779XISSN: Print 1816-3319
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Mutational Analysis of gyrA, gyr B and par C genes Encoding for Fluoroquinolones Resistance in Salmonella typhi and Salmonella paratyphi a Isolated from Blood Culture of Enteric Fever Patients in a Tertiary Care Hospital
M. Banumathy, S. Viji and V. M. Theeba
Page: 6-11 | Received 12 Aug 2023, Published online: 24 Aug 2023
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Abstract
Emergence of multi‐drug resistant Salmonella strains, made the fluoroquinolones like Ciprofloxacin being the drug of choice in the treatment of typhoid feverearlier. But indiscriminate use of fluoroquinolones led to the development of resistance against these agents also. Present study was aimed to study mutational analysis of gyrA, gyr B and par C genes encoding for fluoroquinolones resistance in Salmonella typhi and Salmonella paratyphi A isolated from blood culture of enteric fever patients in a tertiary care hospital. Present study was single‐center, prospective, observational study, conducted in patients with signs and symptoms of enteric fever. Study on molecular characterization of anti‐microbial resistance in Salmonella enterica serovar typhi and paratyphi was carried out. 250 patients of clinically suspected enteric fever cases were analysed. A total of 28 (11.2 %) blood culture positive Salmonella enterica were isolated from 250 blood culture samples, serovars identified were 24 Salmonella typhi (86%) and 4 Salmonella paratyphi A (14%). Male: Female ratio of the patients included in this study was 1.03: 1.Majority of the Salmonella enterica species was isolated from the age group of 21‐30 years (33%), followed by 11‐20 years and 31‐40 years (25% each). The antimicrobial susceptibility pattern of Salmonella typhi showed 100% sensitivity to Chloramphenicol, Cotrimoxazole, Ampicillin, Cefotaxime, Ceftriaxone and Azithromycin whereas 50% of the isolates were resistant to Nalidixic acid, Pefloxacin and Ciprofloxacin. The quinolone resistance was found to be high in S. paratyphi A (50%) than in S. typhi (25%). All the 25% (n = 24), of quinolone resistant S. typhi isolates and 50% (n = 4) of S. paratyphi A isolates were found to be negative for qnrA, qnrB and qnrS which are plasmid mediated quinolone resistance genes. The present study reveals that, typhoidal Salmonellae isolated were mostly resistant to fluoroquinolones, which was due to mutations in the quinolone resistance determining region (QRDR) of gyrA and parC gene.
How to cite this article:
M. Banumathy, S. Viji and V. M. Theeba. Mutational Analysis of gyrA, gyr B and par C genes Encoding for Fluoroquinolones Resistance in Salmonella typhi and Salmonella paratyphi a Isolated from Blood Culture of Enteric Fever Patients in a Tertiary Care Hospital.
DOI: https://doi.org/10.36478/10.59218/makijtm.2023.2.6.11
URL: https://www.makhillpublications.co/view-article/1816-3319/10.59218/makijtm.2023.2.6.11