International Journal of Tropical Medicine
ISSN: Online 1818-779XISSN: Print 1816-3319
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Study to identify Acinetobacter species from various clinical samples and antimicrobial resistance pattern to demonstrate it with special emphasis on molecular characterization of Carbapenemase producing strains at a tertiary hospital
V. M. Theeba, M. Banumathy and S. Viji
Page: 12-17 | Received 13 Aug 2023, Published online: 24 Aug 2023
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Abstract
Background: Acinetobacter is an opportunistic bacterial pathogen primarily associated with Hospital-acquired infections. One of the most striking features of Acinetobacter species is their capacity to produce multiple drug resistant mechanisms against major antibiotic classes. Present study was conducted to study the prevalence of MBL among carbapenem resistant strains of Acinetobacter species in our hospital and to investigate the presence of OXA type beta-lactamases in Meropenem- resistant Acinetobacter isolates using PCR. Material and Methods: Present study was single-center, prospective, observational study, conducted in patients of all groups, who had prolonged hospitalization in the intensive care unit, medical wards, surgical wards, orthopaedic wards, paediatric wards and gynaecology wards. Results: Out of the total 622 samples, 351 (56%) were found to be culture positive. Out of 103 isolates, 59(57%) isolates were between the age group of 51 -60 years. Among them 70 were males and 33 were females with the ratio of 2:1.A.baumannii 100 (97%) was the most common isolate among. The highest sensitivity was for Colistin (100%) and lowest sensitivity for Cefotaxime (15%). 45(62%) isolates were confirmed as ESBL producers. Among the 100 isolates, 20(100%) of them were positive for bla OXA-51 and bla OXA-23 genes. blaVIM coexisted with blaOXA-23 and blaOXA51 in 10(50%) isolates and no isolate was positive for blaIMP. Conclusion: Simultaneous existence of different classes of Carbapenemases is a major problem to encounter with and hence detection methods are required for each of these. In outbreaks, an initial screening of the Carbapenemase producers will help to organize early therapeutic interventions.
How to cite this article:
V. M. Theeba, M. Banumathy and S. Viji. Study to identify Acinetobacter species from various clinical samples and antimicrobial resistance pattern to demonstrate it with special emphasis on molecular characterization of Carbapenemase producing strains at a tertiary hospital.
DOI: https://doi.org/10.36478/10.59218/makijtm.2023.2.12.17
URL: https://www.makhillpublications.co/view-article/1816-3319/10.59218/makijtm.2023.2.12.17