International Journal of Tropical Medicine
ISSN: Online 1818-779XISSN: Print 1816-3319
Abstract
Apoptosis is defined as programmed or controlled cell death which is often regulated. It is as important as cell production. This process is a form of cell suicide which occurs within individual cells. Apoptosis is induced in patients with active tuberculosis by activation of circulating polymorphonuclear neutrophils(PMN). To evaluate the Mycobacterium tuberculosis induced apoptosis in the peripheral blood neutrophils of patients with active tuberculosis. To compare the percentage of apoptotic neutrophils in tuberculosis versus normal controls. The present study included 40 cases of tuberculosis and 10 controls. The study included 4 groups: Group 1: Sputum positive TB. Group 2: Sputum negative, X‐ray positive TB. Group 3: Extra pulmonary tuberculosis. Group 4: Healthy volunteers. Samples collected in EDTA tubes, centrifuged in PCV tubes, buffy coat smears stained with Leishman stain and accretion orange dyes, examined under oil immersion light microscopy and fluorescence microscope at a final magnification of X 1000. The percentage of apoptotic neutrophils were determined by counting the number of neutrophils showing features of apoptosis. The percentage of apoptotic neutrophils were increased in peripheral smears of patients with pulmonary and extra pulmonary tuberculosis. There was no significant increase in apoptotic neutrophils in peripheral smears of healthy volunteers. The Fishers Exact test was used for comparison between the groups and a ‘p’ value of <0.001 was considered statistically significant. Mycobacterium tuberculosis induces apoptosis in peripheral blood neutrophils. Increased circulating apoptotic neutrophils in patients with pulmonary and extra pulmonary tuberculosis correlated positively with disease activity when compared to healthy volunteers.
How to cite this article:
Aparna Narasimha and C. Lalitha. Apoptosis in Peripheral Blood Neutrophils in Patients with Active Tuberculosis: A Case Control Study.
DOI: https://doi.org/10.36478/10.36478/makijtm.2025.1.44.48
URL: https://www.makhillpublications.co/view-article/1816-3319/10.36478/makijtm.2025.1.44.48