International Journal of Tropical Medicine
ISSN: Online 1818-779XISSN: Print 1816-3319
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Abstract
The prevalence of vancomycin‐resistant enterococci (VRE) in hospitals has escalated into a severe threat to patients due to the problem of multiple drug resistance. These strains possess various resistance genes, leading to high‐level inducible or low‐level intrinsic resistance to vancomycin. Infections caused by VRE strains are associated with elevated mortality rates. The study aimed to characterize phenotypically and genotypically VRE strains isolated from diverse clinical samples. 150 enterococcus strains were isolated from different clinical samples. Phenotypic and genotypic methods were used to identify and specify strains. Broth microdilution methods determined the MIC of vancomycin and genotypic characterization was performed using PCR. out of 150 Enterococcus species isolated, were subjected to vancomycin resistance using conventional microbiological methods. The incidence rate of male VRE was 7/11 (63.6%) and female VRE was 4/11 (34.4%). Most VREs were from male patients of different age groups. Of these, 7 were E. faecalis and 4 were E. faecium. When these 11 strains of VRE were tested for the presence of the Van A gene, 9(81.8%) were positive and 2(18.2%) were negative. The present study showed the prevalence of van A genes. These findings have profound and practical implications for healthcare professionals and researchers, equipping them with actionable knowledge to effectively address the growing threat of VRE infections.
How to cite this article:
Trapti Sharma, Madhurendra Singh Rajput and Chaudhary Devendra. Phenotypic Detection and Genotypic Characterization of Vancomycin‐Resistant Enterococci in a Tertiary Care Hospital.
DOI: https://doi.org/10.36478/10.36478/makijtm.2024.3.173.177
URL: https://www.makhillpublications.co/view-article/1816-3319/10.36478/makijtm.2024.3.173.177