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Research Journal of Medical Sciences

ISSN: Online 1993-6095
ISSN: Print 1815-9346
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Appraisal of Modified Staining using Bleach Technique for Detection of AFB in Suspected Cases of Tubercular Lymphadenitis: A Prospective Study from Punjab

Silky Mahajan, Arvind Khajuria and Mukul Chopra
Page: 109-113 | Received 25 Feb 2024, Accepted 12 Apr 2024, Published online: 24 Apr 2024

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https://doi.org/10.36478/10.59218/makrjms.2024.6.109.113

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Abstract

Majority of the studies have been done on sputum and body fluids samples for detecting TB bacilli by using bleach method but very few of them are there on lymph nodes aspirate samples. The present study has been undertaken to find out the efficacy of modified staining using bleach technique in comparison to conventional ZN staining technique for detection of AFB on lymph node aspirates of patients suspected to have tubercular lymphadenitis. The 103 patients of clinically suspected tuberculous lymphadenitis belonging to all the age groups, who were referred for FNAC, were included in this study. The smears, one with conventional ZN technique and the other with bleach concentration technique were examined under oil immersion lens for the presence of AFB. Among the 45 cases diagnosed as tuberculous lymphadenopathy, Granulomatous inflammation with necrosis (68.88%) pattern was the most common one. Reactive hyperplasia was seen in 52(50.49%) cases followed by granulomatous inflammation with necrosis in 31 (30.10%) lymph node aspirate. 24/31 (77.41%) were positive for AFB by conventional ZN technique, while bleach method detected AFB in 26/31 (83.87%) cases. Hence, two cases missed by the conventional ZN staining method were picked up by the bleach method. The incidence of appendageal skin tumors is relatively uncommon. Histopathological study of clinically suspected cases of skin appendageal tumor is a need of an hour.

How to cite this article:

Silky Mahajan, Arvind Khajuria and Mukul Chopra. Appraisal of Modified Staining using Bleach Technique for Detection of AFB in Suspected Cases of Tubercular Lymphadenitis: A Prospective Study from Punjab.
DOI: https://doi.org/10.36478/10.59218/makrjms.2024.6.109.113
URL: https://www.makhillpublications.co/view-article/1815-9346/10.59218/makrjms.2024.6.109.113