files/journal/2022-09-03_18-51-40-000000_599.png

Research Journal of Medical Sciences

ISSN: Online 1993-6095
ISSN: Print 1815-9346
About US
Editors
Issues
Pages
Indexed in
149
Views
15
Downloads

Immunohistochemical Alysis of Ctokeratin 7 and Cytokeratin 20 Expression in Esophageo-Gastrointestinal Epithelial Malignancies

N. Chaitra, Renoy Roshan Edward, B. Kruthi, H.K. Sharath Kumar and G. Nataraju
Page: 32-37 | Received 12 Sep 2023, Published online: 16 Oct 2023

Download Citation
https://doi.org/10.36478/10.59218/makrjms.2023.12.32.37

Full Text Reference XML File PDF File

Abstract

Gastrointestinal malignancies are commonest in the general population with higher incidence of adenocarcinomas. The primary site of origin of adenocarcinomas is difficult to determine, solely by histopathological examination. Thus understanding the site specific immunohistochemical expression of gastrointestinal adenocarcinomas is worthwhile. This study aims to analyse the expression of Cytokeratin 7 and 20 by immunohistochemistry in esophageal, gastric and intestinal carcinomas. This study included 90 specimen, comprising of 20 esophageal, 30 gastric and 40 colonic carcinomas. Routine histopathological examination was done and paraffin blocks were stained with Cytokeratin 7 and 20 immunohistochemistry. Correlation of the study results with anatomic site of origin of tumor was done. Out of 20 esophageal carcinomas, 15 were squamous cell carcinoma and 5 were esophageal adenocarcinoma. Cytokeratin 7 and 20 was negative(Score 0) in 100% cases of squamous cell carcinoma. Majority of the esophageal adenocarcinomas showed score 2+ positive staining for Cytokeratin7 and were negative (Score 0) for cytokeratin 20. 30 gastric carcinomas were included in the study and all the cases were adenocarcinomas. Cytokeratin 7 positivity was noted in 100% of cases of gastric adenocarcinoma and Cytokeratin 20 was found to be positive in 19/30 cases (63%). 39/40 cases (95%) of colonic carcinomas showed Cytokeratin 20 positivity. Esophageal adenocarcinoma showed CK7+/20-, dual positivity favored gastric carcinoma and CK7-/CK20+ expression in colonic adenocarcinoma. We conclude that the property of organ and site specificity of Cytokeratins can be utilised in diagnosing the primary esophageo-gastriointestinal carcinomasand highlights the combined utility of Cytokeratin 7 and 20 immunoexpression.

How to cite this article:

N. Chaitra, Renoy Roshan Edward, B. Kruthi, H.K. Sharath Kumar and G. Nataraju. Immunohistochemical Alysis of Ctokeratin 7 and Cytokeratin 20 Expression in Esophageo-Gastrointestinal Epithelial Malignancies.
DOI: https://doi.org/10.36478/10.59218/makrjms.2023.12.32.37
URL: https://www.makhillpublications.co/view-article/1815-9346/10.59218/makrjms.2023.12.32.37