Research Journal of Medical Sciences
ISSN: Online 1993-6095ISSN: Print 1815-9346
Abstract
Dengue fever is a mosquito‐borne viral infection prevalent in tropical and subtropical regions worldwide, with approximately 390 million infections annually. It poses a significant public health challenge due to its potential for outbreaks and severe complications, such as hemorrhagic fever and shock syndrome, particularly in areas with inadequate vector control measures. The aim of the present study was to analyze the efficacy of filter paper method in detection of Dengue IgM antibody. This cross‐sectional study included 67 samples which were subjected to filter paper method and serum ELISA analysis. The efficacy of filter paper method was determined by calculating sensitivity, specificity, Positive Predictive value and Negative Predictive Value. The study included 67 patients, of which 49.25% were male and 50.75% were female. Of these, there was only one female patient who was older than the male patients in the 0–60 age category, with the majority of patients being between 21 and 30 years old. Of the 67 patients in the study, 50 patients tested Serum Positive, of which, 49 patients also tested Filter Paper Positive. All the 17 patients who tested Serum Negative also tested Filter Paper Negative. 18 patients tested Filter Paper Negative, of which, only 1 patient tested Serum Positive and the remaining 17 patients tested Serum Negative. The study recorded 100 % Specificity and Positive Predictive Value (PPV) each. Sensitivity and Negative Predictive Value were recorded as 98 % and 94.4 % respectively. The filter paper method offers a practical and efficient alternative to ELISA for dengue diagnosis, particularly in resource‐constrained settings, with its simplicity, cost‐effectiveness and ease of use.
How to cite this article:
Sri Lakshmi Gollapalli, Shahjahan Banu, Najma Farheen and Syeda Fahada Zia. A Study on Filter Paper Method vs Elisa in Detection of Dengue Serum IGM Antibody.
DOI: https://doi.org/10.36478/10.36478/makrjms.2024.6.420.423
URL: https://www.makhillpublications.co/view-article/1815-9346/10.36478/makrjms.2024.6.420.423