Research Journal of Medical Sciences
ISSN: Online 1993-6095ISSN: Print 1815-9346
135
Views
15
Downloads
Abstract
In order to identify genes that encode resistance to carbapenems in urinary isolates of Escherichia coli obtained from patients who were hospitalized at a tertiary care centre in Indore, India. A total of 300 consecutive non‐duplicate (one isolate per patient) clinical isolates of Escherichia coli was obtained from urine cultures of hospitalized patients, including those admitted to the medical and surgical intensive care units due to hospital acquired infections, were included in the study. Identification and antibiotic sensitivity assays were performed by standard methods. Polymerase chain reactions (PCR) were utilized to identify the presence of beta‐lactamase‐encoding genes. All of the isolates exhibited complete resistance to carbapenems and cephalosporins of the second and third generations. In vitro susceptibility of each isolate to tigecycline and colistin was one hundred percent. blaNDM‐1 was detected in all of the isolates, and blaOXA‐48 co‐associated with 55% of the isolates. In conclusion, it was observed that urinary isolates of E. coli co‐produced NDM‐1 and OXA‐48 which were highly resistant to the antibiotics. The timely identification of these genes will contribute to effective infection control and prevention measures by restricting the dissemination of these pathogens.
How to cite this article:
Amrita , Ramanath Karicheri and Dhananjay Kumar Pandey. Co‐Production of NDM‐1 and OXA‐48 Carbapenemase, in Urinary Isolates of Escherichia Coli, at a Tertiary Care Centre at Central India.
DOI: https://doi.org/10.36478/10.36478/makrjms.2024.5.675.678
URL: https://www.makhillpublications.co/view-article/1815-9346/10.36478/makrjms.2024.5.675.678