Research Journal of Medical Sciences
ISSN: Online 1993-6095ISSN: Print 1815-9346
251
Views
2
Downloads
Early Detection of Dengue Fever on Day One Using Clinical Criteria and NS1 Antigen Microelisa: A Comparative Study with Immunochromatography
P. Sreelatha, P. Shyam Sunder Rao and B. Nageshwar Rao
Page: 151-155 | Received 20 Aug 2024, Published online: 18 Nov 2024
Full Text Reference XML File PDF File
Abstract
Dengue is prevalent in tropical and subtropical regions, caused by an RNA virus of the Flaviviridae family and transmitted primarily by Aedes aegypti mosquitoes. While most cases are self‐limiting, a small percentage progress to severe forms, including dengue hemorrhagic fever and dengue shock syndrome. This study aims to facilitate early dengue diagnosis, specifically on the first day of fever, using clinical criteria and NS1 antigen detection via MICROELISA, comparing results with immunochromatography for NS1 antigen, IgM and IgG antibodies. With no available vaccine or specific antiviral therapy, early diagnosis on day one is critical for effective patient management. The study included 104 patients presenting with clinical signs of dengue, such as fever, headache, retro‐orbital pain, muscle and joint pain, flushed skin, rash, petechiae and bleeding manifestations. Serum samples were collected from patients on the first day of fever and tested for NS1 antigen using MICROELISA and immunochromatography, along with IgM and IgG antibodies by immunochromatography. Among the 104 serum samples, 46 (44%) tested positive for NS1 antigen via MICROELISA and 37 (35%) were positive by NS1 antigen immunochromatography. Three samples (2%) were positive for IgM antibodies and one was positive for IgG antibodies by immunochromatography. Correlation of MICROELISA with immunochromatography highlighted the value of NS1 antigen MICROELISA in early dengue detection. This study emphasizes the effectiveness of NS1 antigen MICROELISA as a reliable method for the day‐one diagnosis of dengue fever, offering a timely and accurate diagnostic tool essential for early patient management in the absence of a vaccine or specific antiviral treatment. The findings support NS1 antigen MICROELISA as a critical step in improving outcomes for dengue patients through prompt diagnosis and intervention.
How to cite this article:
P. Sreelatha, P. Shyam Sunder Rao and B. Nageshwar Rao. Early Detection of Dengue Fever on Day One Using Clinical Criteria and NS1 Antigen Microelisa: A Comparative Study with Immunochromatography.
DOI: https://doi.org/10.36478/10.36478/makrjms.2024.12.151.155
URL: https://www.makhillpublications.co/view-article/1815-9346/10.36478/makrjms.2024.12.151.155